8/12/2023 0 Comments Cyclic amp signaling pathwayIn cell biology, protein kinase A ( PKA) is a family of enzymes whose activity is dependent on cellular levels of cyclic AMP (cAMP). All rights reserved.CAMP-dependent protein kinase hetero12mer, Sus scrofa Our study may help identifying new genes of interest as potential therapeutic targets or clinical biomarkers for these pathologies and could serve as a guide in future gene therapy studies.Ītrial fibrillation Heart failure Left atria Right atria cAMP-signaling cascade β-Adrenergic pathway.Ĭopyright © 2021 Elsevier Ltd. We give here a general overview of how different pathologies affect the expression of key genes in the cAMP signaling pathway in human right and left atria appendages. However, expression patterns of the genes involved in the atria of healthy and diseased hearts are still unclear. The cyclic AMP signaling pathway is important for atrial function. Different phosphodiesterase (PDE) families showed specific pathology-dependent changes.īy comparing mRNA-expression patterns of the cAMP-signaling cascade related genes in right and left atrial appendages of human hearts and across different pathologies, we show that 1) gene expression is not significantly affected by cardioplegic solution content, 2) it is appropriate to use SR atrial samples as controls, and 3) many genes in the cAMP-signaling cascade are affected in AF and HF but only few of them appear to be chamber (right or left) specific. PDE8A, PI3Kγ and EPAC2 were upregulated in AF. Despite the enormous chamber-dependent variability in the gene-expression changes between pathologies, several distinguishable patterns could be identified. Our results show that right and left atrial appendages in donor hearts or from SR patients have similar expression values except for AC7 and PDE2A. 113 samples were obtained from right atrial appendages (RAA) of patients in sinus rhythm (SR) with or without atrium dilation, paroxysmal atrial fibrillation (AF), persistent AF or heart failure (HF) and left atrial appendages (LAA) from patients in SR or with AF. To obtain a quantitative expression profile of the main genes involved in the cAMP-signaling cascade in human control atria and in different cardiac pathologies.Įxpression of 48 target genes playing a relevant role in the cAMP-signaling cascade was assessed by RT-qPCR. Electronic address: 12 Université Paris-Saclay, Inserm, UMR-S 1180, Châtenay-Malabry, France Institute of Experimental Cardiovascular Research, University Medical Center Hamburg-Eppendorf, Germany German Center for Cardiovascular Research (DZHK), partner site Hamburg/Kiel/Lübeck, Germany. 11 Université Paris-Saclay, Inserm, UMR-S 1180, Châtenay-Malabry, France.of Cardiovascular Surgery, University Heart Center Hamburg, Germany. 10 German Center for Cardiovascular Research (DZHK), partner site Hamburg/Kiel/Lübeck, Germany Dept.9 Institute of Pharmacology, West-German Heart and Vascular Center, Faculty of Medicine, University Duisburg-, Essen, Germany.Internal Medicine II, University Heart Center, University Hospital Regensburg, Germany. 7 Institut Hospitalier Jacques Cartier, Massy, France. 5 Cardiac Surgery Department, Hospital de la Santa Creu i Sant Pau, Spain.3 Université Paris-Saclay, Institut de Chimie des Substances Naturelles, CNRS UPR 2301, Gif-sur-Yvette, France.2 Institute of Experimental Cardiovascular Research, University Medical Center Hamburg-Eppendorf, Germany German Center for Cardiovascular Research (DZHK), partner site Hamburg/Kiel/Lübeck, Germany.1 Université Paris-Saclay, Inserm, UMR-S 1180, Châtenay-Malabry, France.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |